RPA1 and infection: Hitherto, various isothermal amplification techniques coupled with different biosensing systems have been developed for MP infection diagnosis, such as MCDA combined with nanoparticle-based lateral flow biosensor (LFB) (Wang et al., 2019), LAMP combined with LFB (Xiao et al., 2022), and recombinase polymerase amplification (RPA) combined with real-time fluorescent probe (Jiang et al., 2022), incarnating the advantages of sensitivity, specificity, simplicity and rapidity.