There was a markedly high expression of TLR4 in the Dox-HF group (mRNA, 2.17 ± 0.11; protein, 1.68 ± 0.18) compared with the Dox-baseline group (mRNA, 0.83 ± 0.04; protein, 0.54 ± 0.06) (mRNA, P = 0.017; protein, P = 0.009). Here, TLR4 is linked to hydrops fetalis.