High expression of four genes in the six-FRG signature (FANCD2, PTGS2, SLC2A1, and SQLE) were associated with poor prognosis in CCA patients and low expression of the six-FRG other two genes (ACO1 and GOT1) were associated with poor prognosis in CCA patients. The gene discussed is PTGS2; the disease is cholangiocarcinoma.