Four were protective factors (ACO1, PEBP1, GOT1, and CXCL12), and 13 genes were risk factors (FANCD2, MT1G, PTGS2, SQLE, NQO1, SLC1A5, TF, MUC1, HELLS, SLC7A5, HAMP, SLC2A1, and RRM2) in CCA patients. The gene discussed is NQO1; the disease is cholangiocarcinoma.