The mRNA expression levels of 18 m6A regulators (METTL3, METTL16, WTAP, VIRMA, RBM15, CBLL1, YTHDF1, YTHDF3, HNRNPC, FMR1, LRPPRC, HNRNPA2B1, IGFBP3, RBMX1, ELAV1, IGF2BP1, FTO, and ALKBH5) were significantly increased in ESCC compared with normal tissues. This evidence concerns the gene METTL3 and esophageal squamous cell carcinoma.