SMPD1 and cardiomyopathy: Actually, cardiac ceramide content tended to decrease in ASMaseMyh6KO mice and imipramine treated mice as compared to control group, however, this decrease did not reach to the normal levels, thus indicating that HFD-induced cardiomyopathy caused by ASMase overexpression, at least in the context of cardiac lipid overload, is not entirely dependent on ceramide generation.