Accordingly, acetyl-CoA abundance decreased in 3D cultured mouse (4T1 and EMT6.5) and human (MCF7 and MCF10A H-RasV12) breast cancer cells upon CPT1a silencing (Extended Data Figure 6c-e) as well as in 4T1 lung metastases when the mice were treated with etomoxir (40 mg/kg, i.p. injection once a day starting 72 hours before euthanasia 26) (Figure 5c). This evidence concerns the gene CPT1A and breast carcinoma.