Moreover, sphere formation assays revealed that co-transfected with miR-7-5p and pcDNA3.1/KLF4 abolished the miR-7-5p-induced suppression of the tumor sphere-forming capacity measured by quantifying the number and diameter of spheres, and the same trend occurred in the presence of irradiation at a dose of 4 Gy (Fig. 5D–F). This evidence concerns the gene KLF4 and neoplasm.