SOX2 and colorectal carcinoma: Moreover, to clarify the underlying mechanisms governing the regulation of CSC by miR-7-5p in CRC cells, a group of stemness-related proteins were detected by western blot assay, illustrating that overexpressing miR-7-5p remarkably decreased the expression of KLF4, CD133 and SOX2 w/wo irradiation (0 or 4 Gy; Fig. 3E).