To validate that PRDX1 is the dominant target protein for the cell cycle arrest and apoptosis induced by Celastrol and compound 19-048 in colorectal cancer cells, we generated PRDX1-knockdown (gPRDX1) SW620 cells by CRISPR-Cas9 system, with the scrambled negative sgRNA as control (gNS) (Fig. 5a). This evidence concerns the gene PRDX1 and colorectal cancer.