Strikingly, the OE of SIK1, SIK2, or SIK3 in the colon reversed the effect of HG-9-91-01 on TNBS-induced weight change, colitis severity, and TNF-α, IL-12, and IL-10 levels, demonstrating that the anti-inflammatory effect of HG-9-91-01 during colonic inflammation is dependent on all three SIK isoforms (217). This evidence concerns the gene TNF and colitis.