TCRγδ+CD8αα+ IELs are largely exhausted during chronic inflammation, which are mainly considered a guard in the intestinal mucosa and contribute to pathogen restriction and tight regulation of innate and adaptive immune responses.20 TCRγδ+ cells expressing high gut-homing integrins (CD103 and α4β7) are considered ‘inflammatory’ cells due to the enhanced Th1/Th17 cell differentiation after the adoptive transfer.21 To investigate the properties of TCRγδ+CD8αα+ IELs during colitis, we examined the expression of different inflammatory cytokines by flow cytometry. The gene discussed is ITGAE; the disease is colitis.