However, depletion of total neutrophils using anti-Ly6G antibody did not further affect the frequencies and cytokine production (e.g., IFN-γ,·IL-17A, and TNF-α) of TCRγδ+CD8αα+ IELs in colitic Cd177−/− mice compared with those in control IgG2a-treated Cd177−/− mice, indicating that CD177+ neutrophils mainly regulate TCRγδ+CD8αα+ IELs during colitis (Supplementary Figure S2b,c and Figure 1f). This evidence concerns the gene TNF and colitis.