Using FCM, it showed two distinct groups, with approximately 36.2% of cells (accounting for all nucleated cells) classified as abnormal naive lymphocytes, expressing some B-lineage markers (CD19+, cCD79 dim, CD38+, CD123+, CD9part+, CD24part+) and approximately 54.5% of cells (accounting for all nucleated cells) classified as abnormal myeloid primitives that express some markers (CD38, CD64, CD33, CD123dim, CD117, CD11b, CD15, CD13dim), combined with MICM typing are used to diagnose mixed-phenotype acute leukemia(MPAL) (B/M, MLL-AF10, CNS2). This evidence concerns the gene CD38 and mixed phenotype acute leukemia.