To evaluate the role of Cx43-glutamate signaling in the NMO-induced circadian rhythm dysregulation, we treated normal astrocytes with a specific Cx43 hemichannel (Cx43 HC) inhibitor TAT-Gap19 (20 μM) or a glutamate N-methyl-d-aspartate receptor (NMDAR) noncompetitive antagonist DQP-1105 (20 μM). Here, GJA1 is linked to neuromyelitis optica.