ESR1 and non-small cell lung carcinoma: To demonstrate the effect of nobiletin on key targets in NSCLC, we treated A549 cells with different concentrations of nobiletin (0, 10, 20, and 40 μM) for 48 h and detected the mRNA expression of p53, ESR1, MMP9, CXCL8, and PARPA by real-time PCR (Table 4).