Soluble ULBP1 and ULBP3 were not detected in supernatants; ULBP1 is not expressed in these melanoma cells [(López-Cobo et al., 2017) and data not shown], while ULBP3 is mainly recruited to EVs, which makes detection usually more difficult by ELISA testing soluble protein. This evidence concerns the gene ULBP3 and melanoma.