This study suggests that Lactobacillus crispatus can significantly inhibit the proliferation, induce apoptosis and inhibit cell migration of Ect1/E6E7 cells in a time-dependent manner, significantly promote the secretion of Th1 cytokines and inhibit the secretion of Th2 cytokines by Ect1/E6E7 cells, and significantly inhibit the potential cancer promoting genes ABCG2, LIG1, PCNA, ATM, OGG1 and HMGB1 protein expression, but promote the tumor suppressor gene TDG protein expression. Here, HMGB1 is linked to cancer.