The results showed that compared with the control group of cervical squamous cell carcinoma SiHa cells, the expression of differential proteins PCNA, ATM, LIG1 and HMGB1 in Ect1/E6E7 cells decreased significantly, while the expression of TDG and OGG1 proteins increased significantly. The gene discussed is HMGB1; the disease is cervical squamous cell carcinoma.