Next, the CD68+CD163+ (M2-like, component of SIA) cells had high expression of CD14, CD16, ITGAX (CD11c) and CD86 in all three scRNA datasets (Lung, Melanoma, CRC), thus supporting an origin from ‘intermediate’ monocytes,57, 58, 59, 60, 61, 62 although we cannot exclude the increase of the expression of these proteins as a response to local (tissue) stimuli. This evidence concerns the gene ITGAX and melanoma.