The CD68−CD163+ cells had CD14 in all datasets and no (in CRC and Melanoma) or low (Lung) CD16 expression, which indicates an origin from ‘classical’ monocytes.58, 59, 60, 61, 62, 63 Finally, CD68+CD163− (M1-like) cells had low (Lung) or no (CRC and Melanoma) expression of both CD14 and CD16, thus lacking monocyte characteristics and suggesting their origin from tissue-resident macrophages or higher differentiation stage of blood-derived monocytes. Here, CD68 is linked to melanoma.