CTLA4 and melanoma: To enable more accurate signature estimation, we next analysed single-cell sequencing data from melanoma patients treated with anti-PD1 and/or anti-CTLA4 (n = 48)48 and computed SIA using single cell gene expression levels of CD8A to define CD8+ cells and a combination of the expression of CD68 and either CD163, C1QA, C1QB or C1QC to define M2-like macrophages.