S4C), as well as with Western blot measurement of mTORC1 target phospho-S6 ribosomal protein (phospho-S6235/236) following stable lentiviral infection of a short hairpin RNA (shRNA) targeting PIP4K2A (shPIP4K2A) for 72 hours (0.64-fold of control; t test, P = 0.006; Fig. 4D). Here, RPS6 is linked to infection.