TFEB transcription activation is a biomarker of loss of mTORC1 anabolic activity, and we observed an up-regulation of three of four tested TFEB target genes (CTSD, NEU1, HEXA, and CTSF) following siPIP4K2A depletion in LNCaP cells in AD (Fig. 4E). Here, CTSF is linked to Alzheimer disease.