Meanwhile, ML385, similar to ANI, could also inhibit the protective effects of SIV on ALI in vitro and in vivo, such as increasing the ROS levels in cells, increasing the inflammatory cytokines levels and neutrophil percentage in BALF, decreasing the SOD and GSH-Px levels in rat lung tissues, and aggravating the oxidative stress and inflammatory responses. Here, SOD1 is linked to acute respiratory distress syndrome.