PXN and melanoma: We generated mouse YUMM1.7 melanoma tumors either expressing the wildtype, the phosphomimetic (Y118E), or the non-phosphorylatable (Y118F) version of mammalian Paxillin and fused the Paxillin mutants to a self-cleavable GFP (T2A-GFP); thus, GFP localization is uncoupled from Paxillin localization in these cells.