PXN and melanoma: Using mouse melanoma tumors expressing either wildtype, phosphomimetic (Y118E), or non-phosphorylatable (Y118F) versions of mammalian Paxillin, we immunoprecipitated Paxillin and found there is no difference in the level of C3G interactions with Paxillin when cells are expressing wildtype, Y118E, or Y118F-Paxillin in vivo or in vitro (Fig. S5, D, E, G, and H).