Additionally, 914 differentially expressed proteins were identified that were expressed five-fold or higher in the FLCNPOS and FLCNNEG cells.36 We aimed to utilize a similar approach in which we generated a NDUFA4L2 knockout line in a human renal cell carcinoma cell line (RCC4-KO-643) and then performed immunoprecipitations of RCC4-P and RCC4-KO-643 with an antibody to NDUFA4L2 to identify potential interacting proteins of NDUFA4L2 to gain further insight as to how NDUFA4L2 functions (Figure 2c, 2d). The gene discussed is COXFA4L2; the disease is hereditary clear cell renal cell carcinoma.