Additionally, 914 differentially expressed proteins were identified that were expressed five-fold or higher in the FLCNPOS and FLCNNEG cells.36 We aimed to utilize a similar approach in which we generated a NDUFA4L2 knockout line in a human renal cell carcinoma cell line (RCC4-KO-643) and then performed immunoprecipitations of RCC4-P and RCC4-KO-643 with an antibody to NDUFA4L2 to identify potential interacting proteins of NDUFA4L2 to gain further insight as to how NDUFA4L2 functions (Figure 2c, 2d). This evidence concerns the gene SLC49A4 and renal cell carcinoma.