The VAF value of mutations in ECCC was low like that in OCCC, the timing order of the mutation events arose in the tumor was different, and mutations such as TP53, PPP2R1A, HIF1A, KRAS, SPOP, MAP3K1, and PIK3CA are almost concurrent by the similar VAF value of mutation. The gene discussed is MAP3K1; the disease is neoplasm.