Compared with the DIT, GO analysis demonstrated that all regulated alternative splicing genes (RASGs) were mainly enriched in atherosclerosis-related pathways like small GTPase-mediated signal transduction, signal transduction, actin cytoskeleton organization, negative regulation of the I-κB kinase/NF-κB cascade, regulation of cell shape, transcription regulation and other functional pathways (Fig. 2D). This evidence concerns the gene NFKB1 and atherosclerosis.