Stimulation with Th1 cytokine mixes (IL-17A, IL-6, IL-22, IL-1α ± TNF-α) for Ps induction and Th2 mixes (IL-4, IL-13 ± IL-31) for AD induction resulted in loss of filaggrin (FLG) and reduction of cytokeratin-10 (CK10) levels while involucrin (IVL) was decreased in AD models but less affected in Ps substitutes. The gene discussed is FLG; the disease is Alzheimer disease.