FOXO1 and posterior cortical atrophy: To further verify whether SGK2 phosphorylates T24 and S319 of FOXO1 in PCa and promotes the nuclear exclusion of FOXO1, we established a stable knockdown of FOXO1 in PC3 and DU145 cell lines, and transfected plasmids expressing threonine-alanine (T24A) and/or serine-alanine (S319A) mutant FOXO1 (Supplementary Fig. S9C).