The CV risk factors, ageing and mild dyslipidaemia, initiate early vascular changes and predispose to atherosclerosis, the main cause of CVD.36 To assess the response to these early vascular changes, we used flow cytometry to dissect changes in CD55+, CXCL14+, and LOX+ fibroblasts between young and aged mice, and between normolipidaemic wild-type mice and Ldlr KO mice on a chow diet to induce mild hypercholesterolaemia. The gene discussed is LDLR; the disease is Hypercholesterolemia.