In this study, we found that the m6A RNA methylation eraser FTO was dramatically down-regulated in glioma samples and cell lines, particularly in the intermediate and core regions and hypoxia-challenged glioma cells.In vitro, FTO overexpression inhibited the hypoxia-induced capacities of glioma cells to proliferate, migrate and invade and decreased the percentage of cells with m6A RNA methylation.In vivo, FTO overexpression inhibited tumor growth in the xenograft model and decreased the protein levels of migration markers, including Vimentin and Twist. The gene discussed is TWIST1; the disease is central nervous system cancer.