Hence, in the present study, we have systematically analyzed the expression patterns, single-cell levels in human tissue, expression levels in cell subpopulations and gene expression of CD276 in different cell types of different brain tissues; moreover, the expression profile of CD276 in 33 types of cancer, the prognostic value of cancer patients, epigenetic alterations, DNA methylation levels, and the association between CD276 and immune-activating genes, immunosuppressive genes, chemokines, chemokine receptors and immune checkpoints have been investigated. The gene discussed is CD276; the disease is cancer.