In the present study, we used single-cell analysis and bioinformatics methods to investigate CD276 expression patterns, single-cell levels in human tissues, expression levels in cell subpopulations, and gene expression in different cell types in different brain tissues, as well as the expression of CD276 in various types of cancer and its impact on patient prognosis, epigenetic alterations, DNA methylation, GSEA analysis and immune cell infiltration levels. Here, CD276 is linked to cancer.