Then, we further employed RIN1, a RBPJ transcription complex inhibitor (which disrupts the interaction between RBPJ and SHARP and inhibits Notch pathway6), to reversely verify the role of RBPJ, and found that RIN1 inhibited PD-1 expression on CD8+ T cells from subcutaneous tumors of mouse with HCC (Supplementary Fig. 2f). This evidence concerns the gene SPEN and hepatocellular carcinoma.