We next investigated the relationship between RBPJ expression and the malignancy of HCC cell itself, and found that RBPJ expression in HCC was higher than that in paired paracancerous tissues (Supplementary Fig. 13a, b), and RBPJ expression was higher in CNLC III + IV patients than in CNLC I + II patients (Supplementary Fig. 13c). This evidence concerns the gene RBPJ and hepatocellular carcinoma.