The anti-tumor immune response mediated by CD8+ T cells is limited by various mechanisms: high expression of immune regulatory molecules (such as PD-1, PD-L1, IDO1, etc.)in leukocytes or tumor cells, physicochemical imbalances of microenvironment (such as low pH, hypoxia, and amino acid metabolism disorder), metabolic competition with tumor cells, and lack of CD4+ T cell help2. The gene discussed is CD4; the disease is neoplasm.