EIF4E and infection: To evaluate the effect of the eIF4E1 Region I modification on the virus resistance, we used four potyviruses—pepper mottle virus (PepMoV) and three different isolates of potato virus Y (PVY)—to challenge the plants homozygous at the three editing stages (Figure 1a) and quantified the viral accumulation by ELISA 21 days after infection (Table 1).