Moreover, in contrast, to other works that use FBS, insulin at micromolar concentrations, glucose concentrations above 10 mM and dexamethasone concentrations superior to 100 nM, which may affect insulin action (30, 31, 52–54), the model herein developed represents a more physiological system that can be used in the future to induce NAFLD and study disease mechanisms. Here, INS is linked to metabolic dysfunction-associated steatotic liver disease.