Finally, combined with the results of molecular docking, we did immunofluorescence of TNF-α in AC16 cells and heart tissues after modeling, and found that the fluorescence intensity of TNF-α in AC16 cells after NG-R1 treatment was significantly reduced compared with the LPS group, and the fluorescence intensity of TNF-α in heart tissues after NGR1 treatment was also significantly reduced compared with the sepsis group. This evidence concerns the gene RTN4R and Sepsis.