In addition, the high-grade (733.9 ± 70.71) vs low-grade (435.1 ± 31.20) (P=0.0007), metastatic (897.4 ± 79.2) vs non-metastatic (507.2 ± 40.78) (P=0.0002) and recurrent (937.2 ± 98.38) vs non-recurrent (510.5 ± 42.35) (P < 0.0001) osteosarcoma tumors expressed statistically significant amount of IGF-1 compared to their opposite counterparts (Figure 4D). The gene discussed is IGF1; the disease is osteosarcoma.