Mutations in ADAMTS20, ARHGAP10, OR1L4, PDS5A, and RPLP0 were only found in mixed germ cell tumors, while mutations in B3GNT8, CAPN7, FAT4, GRK1, TACC2 and TRAM1L1 were only observed in embryonal carcinoma, and their mutation frequency was around 2%. The gene discussed is B3GNT8; the disease is embryonal carcinoma.