To identify the dominant cellular sources responsible for the upregulation of CCL5 in eWAT by obesity, we detected the expression of CCL5 in CD4+ T cells, CD8+ T cells, macrophages, and adipose tissue stem cells (ASCs) in eWAT from lean and obese mice by flow cytometry. The gene discussed is CD4; the disease is obesity due to melanocortin 4 receptor deficiency.