We document marked decrease in BTLA expression, something which has been documented in the literature, and maximal expression of both TIM3 and LAG3 following stimulation and these molecules, along with PD1, on γδ T-cells have been shown to associate with earlier relapse and shorter overall survival in melanoma patients suggesting these molecules may play a role in Vδ2+ T-cell regulation (17, 93). Here, PDCD1 is linked to melanoma.