We then obtained representative pictures of CD69+ EVs from a GVHD patient’s serum EVs captured by anti-CD9-conjugated latex beads, assessed by STORM and TEM analysis: the pictures show a diameter range of about 100 nm for CD69+ EVs, confirming that they belong to the nano EV fraction (Figures 3B, C). Here, CD9 is linked to graft versus host disease.