In order to assess whether prevention of T1D in AID-fed NOD mice is linked to restoration of GB integrity, we performed a gut permeability test (FITC-dextran) and measured by RT-qPCR the expression of mRNA transcripts of tight junction proteins and analyzed different biomarkers of mucus layer integrity (mucus layer thickness, percentage of GC, mRNA expression levels of mucins) in the intestine of AID vs STD diet-fed NOD mice. The gene discussed is AICDA; the disease is type 1 diabetes mellitus.