We here employed a new set of patients to investigate the methylation level of a novel marker, STAP1, as well as AHNAK, in the PBMC and T cells also in different course samples of hepatitis B. We obtained a similar result for AHNAK. In this study, we investigated the methylation of STAP1 and found that the methylation of STAP1 was negatively correlated with the methylation of AHNAK. Although AHNAK can distinguish hepatitis B patients to a certain extent in the methylation, the combination of these two markers can achieve better performance. The gene discussed is AHNAK; the disease is hepatitis B virus infection.