A further two-paired comparison of STAP1 methylation levels demonstrated that there existed significant differences between NC and DCLC and HCC (stage 0, A, B, C), between CHB and DCLC and HCC, and between CLC and DCLC, stage 0 HCC, and stage C HCC in the PBMC. This evidence concerns the gene STAP1 and hepatocellular carcinoma.