To validate the authenticity of mRNA sequencing results, seven genes related to immune defense were chosen for the qRT-PCR analysis (four up regulated genes and three down regulated genes), including trypsin-like, T-cell leukemia homeobox protein 3-like, C-type lectin 3, Toll interacting protein, caspase-1-like, glutathione peroxidase, and heat shock protein 90, aiming to investigate the expression changes of these genes after DIV1 infection. This evidence concerns the gene TOLLIP and infection.