DND1 and Infertility: In this study, we report the targeted mutagenesis of the dnd1 gene in zebrafish by using CRISPR/Cas9 genome editing technology to achieve complete sterility in dnd1-gene-knockout non-transgenic zebrafish and transgenic fluorescent zebrafish, as the model for the infertility control in farmed ornamental fish and transgenic fish, especially in transgenic fluorescent ornamental fish.