Therefore, CRISPR/Cas9 technology had been applied in genome editing of several fish species such as Atlantic salmon, medaka, sterlet, and rainbow trout to target dnd1 to achieve infertility (Wargelius et al., 2016; Sawamura et al., 2017; Baloch et al., 2019; Baloch et al., 2021; Fujihara et al., 2022). The gene discussed is DND1; the disease is Infertility.