WT1 and type 1 diabetes mellitus: Another early investigation on a larger cohort, this time based on uEVs isolated by a differential centrifugation method (Kalani et al., 2013) reported elevated levels of Wilm’s Tumor-1 (WT1) in the uEVs of T1D with proteinuria compared with patients without proteinuria, whereas in the non-diabetic control group WT1 expression was absent.