The same compounds were then assayed at a single concentration of 30 μM (we initially expected that, having designed the compounds to bind to P2X4, they would likely be less potent at P2X7, and need to be applied at a higher concentration to observe an effect) on 1321N1 astrocytoma cells stably expressing human P2X7. The gene discussed is P2RX4; the disease is astrocytoma (excluding glioblastoma).