To assess the link between mTORC1, refeeding, and tumour initiation in ISC, we administered rapamycin to AL and refed Apcloxp/loxp: Villin-CreERT2 and Apcloxp/loxp; Lgr5-EGFP-IRES-CreERT2 mice (Fig. 6a, Extended Data Fig. 6a). Here, LGR5 is linked to neoplasm.