GH1 and Anxiety: To further probe how GABACeA participated in anxiety-like behaviors in acute pain, we employed the chemogenetic inhibitory rAAV-VGAT1-hM4D(Gi)-mCherry-WPRE-hGH-pA (AAV-VGAT1-hM4Di-mCherry) virus under the regulation of a VGAT1 promoter and the intraperitoneal injection of its ligand clozapine-N-oxide (CNO, i.p., 5 mg/kg) to selectively inhibit GABACeA neurons in the CFA 1D mice (Figure 3A).