The results demonstrated that IFIT1/IFIT3+ T cells were correlated with accumulation of FOXP3+ Tregs in tumor nest of lymph node metastatic (Figure S4a,b, Supporting Information).To further explore the dynamics of T cells in ESCC, we use Monocle2 to inferr the trajectories of T cells from differential expressed profiles and estimated their relative pseudo‐times along trajectories. The gene discussed is IFIT1; the disease is esophageal squamous cell carcinoma.