Given the relevance of the large HSP40 co-chaperone family on TDP-43 proteinopathies54,58,59, we characterized PLD phase separation in the presence of representative members of the different J-domain containing (JDP) subfamilies: DNAJA2 (class A), which is a potent tau aggregation inhibitor;60 DNAJB1 and DNAJB4 (class B), which promote the clearance of TDP-43 deposits;54 and DNAJC7 (class C), which is considered an ALS gene58 (Supplementary Fig. 17). Here, DNAJB4 is linked to amyotrophic lateral sclerosis.