Figure 9A, B revealed simple nucleotide variation of risk score groups in BLCA cases, suggesting that the 20 genes with the highest mutation rate in BLCA were TP53, TTN, KMT2D, MUC16, ARID1A, KDM6A, PIK3CA, SYNE1, RB1, FGFR3, HMCN1, KMT2C, RYR2, MACF1, EP300, FLG, FAT4, STAG2, ATM and OBSCN. The gene discussed is FLG; the disease is bladder transitional cell carcinoma.