Single cell transcriptomic analysis of human NASH samples showed that injured hepatocytes stimulate myofibroblast differentiation and activation through TGFβ-1/2, Sonic hedgehog, and PDGFRBB signaling, which implicates the interplay between hepatocytes and activated fibroblasts in development of NASH-induced HCC.100. This evidence concerns the gene TGFB1 and metabolic dysfunction-associated steatohepatitis.