Additionally, western blot analysis revealed that inhibition of HK2 expression showed significant effects on L1‐mediated upregulation of VEGFA, MMP2, and MMP9 expression in glioma cells (Fig. 6D,E), suggesting that L1 may promote VM formation and tumor invasion via L1/HK2 cascade‐mediated regulation of VEGFA, MMP2, and MMP9 expression in glioma cells. This evidence concerns the gene HK2 and central nervous system cancer.