To obtain further evidence that the miR‐143‐3p‐regulated HK2 is the downstream regulator of L1‐mediated VM formation and tumor invasion in glioma, we next detected HK2 expression in the L1‐overexpressing and control groups of different glioma cell lines with or without miR‐143‐3p mimic transfection. This evidence concerns the gene HK2 and neoplasm.