Our results showed that inactivation of AKT signaling markedly decreased the expression levels of VEGFA, MMP2, and MMP9 in the U87, T98, and GBM1 glioma cell lines even after treatment with the miR‐143‐3p inhibitor (Fig. 7H), suggesting that the PI3K/AKT signaling pathway may be the key downstream factor of L1/HK2 cascade‐mediated VM formation process. Here, HK2 is linked to central nervous system cancer.