TLR4 and infection: At 3 h post infection the TLR4 level was 180% which increased significantly at 6 h (220%, p < 0.0001), 12 h (297%, p < 0.0001) and reached to the level 312 (p < 0.01) at 24 h post infection (Fig. 5) when compared to the level expressed at 0 h post JEV infection.