TLR4 and infection: At 3 h post infection the TLR4 level was increased to 253% which increased significantly at 6 h (506%, p < 0.0001), 12 h (716%, p < 0.0001) and reached to the level 823% (p < 0.0001) at 24 h post infection (Fig. 6) when compared to the level expressed at 0 h post JEV infection.